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Research Paper

Vol. 8 No. 1 (2026): Continuous Flow

Silver(I) tenoxicam complex: In vitro valuation of the antiproliferative activity over B16 melanoma cell line using electrochemotherapy

DOI
https://doi.org/10.52466/ijamb.v8i1.155
Published
2026-07-03

Abstract

Cancer is one of the leading causes of mortality and melanoma is one of the deadliest skin cancers. However, it is a potentially curable condition if diagnosis and treatment occur early. Electrochemotherapy is a therapeutic modality for local control against various types of neoplasia. It is a technique that uses the combination of electroporation, which allows the entry of molecules generally not permeable to the plasma membrane through electrical pulses, and chemotherapy drugs. The objective of this work is the synthesis and characterization of a silver metal complex, using tenoxicam (Ag-TNX) as a ligand, and to evaluate the antiproliferative effects on melanoma tumor cells in vitro, replacing conventional chemotherapy drugs used by the electrochemotherapy technique. Complex characterization tests were carried out, such as FTIR, thermogravimetric analysis and elemental analysis, as well a cytotoxicity tests, application of electroporation associated with the Ag-TNX complex in vitro and determination of absorption of the agent through the Franz cell. The Ag-TNX agent showed antibacterial activity over S. aureus ATCC 25924, E. coli EC 958, E. coli BR43, and P. aeruginosa ATCC 27853, and superior antiproliferative effect in the application of electroporation compared to the isolated Ag-TNX, demonstrating promising results for future expansion of therapeutic arsenal of antineoplastic and antibacterial agents.

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