Effects of grape seed extract on properties of type I collagen scaffolds

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Claudio Fernandes Garcia
Virginia C. A. Martins
Ana M. G. Plepis



To obtain a material with potential for use in tissue engineering, anionic collagen was obtained from porcine serosa (S) and bovine tendon (T) by alkaline hydrolysis for 72h. Part of this collagen was mixed with water to obtain 4 % (weight/weight) collagen suspension and part was solubilized in acetic acid pH 3.5 to obtain 1.5% (w/w) gel. The suspensions were mixed with their respective gels (2:1) (suspension: gel) and grape seed extract, whose main product is proanthocyanidin, was added at concentrations of 0.03% and 0.5%, thus obtaining the scaffolds SC (serosa collagen suspension and gel), TC (tendon collagen suspension and gel), SCP003 (SC with 0.03% extract), TCP003 (TC with 0.03% extract), SCP05 (SC with 0.5% extract added) and TCP05 (TC with 0.5% extract). The materials were analyzed by differential scanning calorimetry (DSC), scanning electron microscopy (SEM) and characterized by phosphate buffered saline absorption assay and in vitro biological stability assay. By DSC it is observed that the addition of 0.5% of extract increases the denaturation temperature (Td) of collagen, indicating that at this concentration the extract acts as polymer crosslinking agent. SEM shows disorganized cross-section pores in all scaffolds, not exceeding 130 ?m. Absorption and degradation assays indicated that the addition of 0.5% extract increases the absorption of phosphate buffered saline (PBS) by the scaffolds and decreases the degradation percentage by collagenase. These results suggests that the scaffolds can be used for different applications, e.g. as hemostatic agent.



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How to Cite
Garcia, C. F., Martins, V. C. A., & Plepis, A. M. G. (2020). Effects of grape seed extract on properties of type I collagen scaffolds. International Journal of Advances in Medical Biotechnology - IJAMB, 2(2), 02-10. https://doi.org/10.25061/2595-3931/IJAMB/2019.v2i2.29
Meeting of Natural Polymers - EPNAT
Author Biography

Virginia C. A. Martins, São Carlos Institute of Chemistry (IQSC), University of São Paulo (USP), São Carlos

Instituto de Química de São Carlos (IQSC), Universidade de São Paulo (USP), São Carlos


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