International Journal of Advances in Medical Biotechnology - IJAMB https://journalamb.com/index.php/jamb <p><strong>International Journal of Advances in Medical Biotechnology (IJAMB)</strong> is a peer-reviewed open access journal which provides a forum for discussion and dissemination of studies in Medical Sciences and Biotechnology fields. As a scientific publisher, IJAMB will focus on the dissemination of Original Papers, Reviews, Mini-reviews, Perspectives, Emerging Technologies and Spotlights relating scientific discoveries and inventions. The submitted papers should indicate the novelty and significant advances to the current state of knowledge and understanding of the proposed topic.</p><p>In this context, the IJAMB emerged as a means of sharing new knowledge oriented towards to the future which is related to the most relevant advances on Medical Sciences and Biotechnology fields. Therefore, the topics of interest to the current journal include: Regenerative Medicine; Biomembranes and Biopolimers; Slow delivery systems of drugs; Medical devices; Medical chemistry organic and inorganic; 3D structures; Growth factors; Tissue Engineering; Electronic technologies for health applications and papers focusing on biological, physiological and pharmacological modelling molecules.</p><p>As editorial guidance, the journal policy is based on the best practices of Brazilian and international scientific journals. Thus, the evaluation process adopted by the Journal is to publish peer-reviewed research which guarantees the confidentiality of authors and reviewers.</p><p>The Editorial Board is committed to build up a journal with international recognition and adequate indexation based on international and national data. We consider to analize content articles as commissioned, but the Journal is also open to welcome new ideas. Please contact the Editorial Office (ijamb@uniara.com.br) with your proposal; once a proposal is accepted or commissioned, detailed format guidelines will be provided.</p> International Journal of Advances in Medical Biotechnology (IJAMB) en-US International Journal of Advances in Medical Biotechnology - IJAMB 2595-3931 <p>Papers may not be offered for publication elsewhere whilst under consideration by the <em>International Journal of Advances in Medical Biotechnology - IJAMB</em>. The corresponding author must obtain the consent of all co-authors to the submission of the paper. Papers accepted by the Journal for publication may not be published elsewhere without the permission of the JOURNAL and acknowledgement of the original source of the publication.</p> Biotechnology applied for sustainable development: social responsibility in the Industry 4.0 https://journalamb.com/index.php/jamb/article/view/38 <p>This research was conducted from a review of bibliographic content on Biotechnology, sustainable development, social responsibility and Industry 4.0. The goal endows the understanding of the role of Biotechnology as a science in sustainable development in this historical phase experienced by humanity, the Fourth Industrial Revolution, verifying what would be the social responsibility of Industry 4.0 in this context. Dialectical and historical methods were used to systematize the obtained data. The importance of maintaining the environmental balance through sustainable practices in the daily life of Industry 4.0 has been demonstrated to comply with the constitutional principle of the social function of property. However, in order to achieve sustainable development, the economic and social aspects, besides the environmental, must be considered. The relevance of Biotechnology in this process has been proven as a driving force for sustainable development. It is hoped with this research to mobilize the academic community and the society in the fight against environmental degradation, bringing knowledge about the role of Biotechnology in this process, in the context of Industry 4.0, and demonstrating the need for companies, professionals and governments to adapt to this new and unknown reality in order to face the problems that are already emerging, always taking into consideration the protection of human rights, especially the healthy and balanced environment, safety, life and dignity of the human person.</p> Antonio Carlos Massabni Oreonnilda de Souza Copyright (c) 2019 International Journal of Advances in Medical Biotechnology - IJAMB 2020-08-03 2020-08-03 2 2 37 44 10.25061/2595-3931/IJAMB/2019.v2i2.38 Biotechnology and Industry 4.0: The professionals of the future https://journalamb.com/index.php/jamb/article/view/39 <p>The process of developing technological research is being carried out beyond organizational boundaries, especially organizations that make intensive use of knowledge, such as Biotechnology. Considerable progress has been made in recent years in reducing costs and increasing the ease of gene sequencing and, ultimately, in activating or editing genes. In this context, discussions have been gaining prominence around Industry 4.0, in which new business models and intelligent processes for Biotechnology are evidenced. However, the challenges regarding the training and education of professionals are discussed, emphasizing the importance of a multidisciplinary education, unlike the more traditional nature of education in the areas of biology and sciences. These challenges can be partially transposed by strengthening the partnerships of universities and research centers with companies, in order to materialize in common projects, the demands of industries and the possibilities of transforming research projects into final products available to society. This paper presents a study on the impact of Industry 4.0 technologies on Biotechnology, and also presents the prospect of future professions with the influence of Industry 4.0. The results show that technologies such as artificial intelligence, robotics and 3D printing are promising in the development and advancement of Biotechnology. New automated laboratories are under development. Also, regulatory issues require a great deal of study, and business models will need to be more efficient to generate the results needed for the development of new drugs, food, and research of new products related to health.</p> Antonio Carlos Massabni Gilson José da Silva Copyright (c) 2020 International Journal of Advances in Medical Biotechnology - IJAMB 2019-10-01 2019-10-01 2 2 45 53 10.25061/2595-3931/IJAMB/2019.v2i2.39 Biotechnology and vaccines https://journalamb.com/index.php/jamb/article/view/40 <p>Biotechnology has demonstrated its importance for health development, especially in the discovery of new drugs and production of vaccines. On account of the occurrence of many diseases that have killed millions of people in the world, vaccines were developed to control infections and prevent diseases caused by viruses, bacteria, protozoans and fungi, and even eradicate them, as is the case of smallpox. Vaccines can be of first, second and third generation. Currently, vaccine manufacturing can be directed to the use of DNA containing the gene that encodes an antigenic protein. The present work is a literature review, with the objective to present the first-, second- and third-generation vaccines, as well as to make an analysis of the use of these vaccines in Brazil.</p> Antonio Carlos Massabni Marco Antonio da Costa Borges Copyright (c) 2020 International Journal of Advances in Medical Biotechnology - IJAMB 2020-02-28 2020-02-28 2 2 54 61 10.25061/2595-3931/IJAMB/2019.v2i2.40 The Fourth Industrial Revolution and the disruptive technologies: implications in work relations https://journalamb.com/index.php/jamb/article/view/41 <p>This research covers an analysis of the essential aspects involving the Fourth Industrial Revolution, disruptive technologies and their consequences on work relations. Using literature reviews, analysing cases of companies that failed and others that remodeled themselves to survive the yearnings of the technological age, it was possible to obtain important results. The research was conducted in three parts: 1. historical contextualization guiding the reader on the main aspects and peculiarities of the Fourth Industrial Revolution; 2. definition, application and some examples of disruptive technologies; 3. confirmation of the impact of these technologies on work relations. The methods used were: hermeneutics, privileging theoretical studies and analysis of documents and texts and the deductive method, starting from existing laws and theories for the development of a logical reasoning to explain the central problem. Negative impacts of mass unemployment due to the replacement of human labor by highly technological machines cannot be stimated. These machines are part of what has been called disruptive technology, i.e., a product or innovative servant destabilizes competition, overcoming it in such a way that it promotes the rupture of existing models, ruining them. Professionals will be called to fill new jobs, with skills and competencies for Industry 4.0, whose interaction between man and machine will be essential. Use of big data in quality control, robots, fully automated vehicles, 3D printers in production lines, among other activities are examples of work demands.</p> Antonio Carlos Massabni Leiraud Hilkner de Souza Copyright (c) 2020 International Journal of Advances in Medical Biotechnology - IJAMB 2019-10-01 2019-10-01 2 2 62 68 10.25061/2595-3931/IJAMB/2019.v2i2.41 The importance of Biotechnology in the health area: historical overview and future perspectives https://journalamb.com/index.php/jamb/article/view/42 <p><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">A biotecnologia, em sentido amplo, compreende a manipulação de microrganismos, plantas e animais, com a proposta de obter processos e produtos de interesse da sociedade. </span><span style="vertical-align: inherit;">Embora o termo tenha surgido no início do século XX, os processos biotecnológicos estão presentes desde os tempos antigos na produção de alimentos e bebidas fermentados. </span><span style="vertical-align: inherit;">A biotecnologia também está presente desde o início da medicina. </span><span style="vertical-align: inherit;">A inalação de pó de feridas de varíola humana secas ao sol ou a secreção do úbere de vacas contaminadas com varíola bovina são exemplos de técnicas antigas usadas como métodos de imunização da doença. </span><span style="vertical-align: inherit;">Nesse sentido, muitos tratamentos surgiram ao longo do tempo, como vacinas, produção de penicilina, o primeiro antibiótico e produção de insulina por DNA recombinante, </span><span style="vertical-align: inherit;">que revolucionaram a Medicina e possibilitaram uma melhor qualidade de vida para os seres humanos. </span><span style="vertical-align: inherit;">Muitos produtos e materiais da Biotecnologia ainda estão surgindo, como andaimes em Medicina Regenerativa para recuperação de tecido ósseo, tubos de celulose bacteriana usados como vasos sanguíneos artificiais, células-tronco pluripotentes induzidas e sistemas microfisiológicos, que prometem melhorar ainda mais a medicina moderna e fornecer melhores técnicas e técnicas. terapias para vários tratamentos.</span></span></p> Jhonatan Miguel Silva Antonio Carlos Massabni Copyright (c) 2019 International Journal of Advances in Medical Biotechnology - IJAMB 2019-10-01 2019-10-01 2 2 69 74 10.25061/2595-3931/IJAMB/2019.v2i2.42 Special session in honor of Antonio Carlos Massabni https://journalamb.com/index.php/jamb/article/view/52 <p>The present issue of International Journal of Advances in Medical Biotechnology (IJAMB) introduces a set of five papers elaborated by Prof. Antonio Carlos Massabni and students that have attended a graduate course nominated Seminars of Integration from the Postgraduate Program in Biotechnology of the University of Araraquara (UNIARA), Araraquara, Brazil. The authors have focused on the impact of Industry 4.0 technologies on Biotechnology as well as the chance for future professionals and the work relations in this context. In other papers, the importance of biotechnology in the development of vaccines, antibiotics, hormones and scaffolds for regenerative medicine are discussed. This issue is dedicated in honor of Prof. Massabni for his commitment with teaching, research and administrative duties.</p> Vera Regina Leopoldo Constantino Pedro Paulo Corbi Copyright (c) 2020 International Journal of Advances in Medical Biotechnology - IJAMB 2019-10-01 2019-10-01 2 2 36 36 10.25061/2595-3931/IJAMB/2019.v2i2.52 Abstracts of the 1st Meeting on Natural Polymers - EPNAT https://journalamb.com/index.php/jamb/article/view/44 <div><div class="_3U2q6dcdZCrTrR_42Nxby JWNdg1hee9_Rz6bIGvG1c allowTextSelection"><div><div class="rps_d519"><div><div class="x_gmail_quote"><div dir="ltr">MEETING OF NATURAL POLYMERS - EPNAT<br />UNIARA - USP - UNIFESP - UNESP<br /><br />The 1st Meeting of Natural Polymers took place on August 5th and 6th, 2019, in the city of Araraquara - São Paulo, and had as main theme "Applications and Perspectives."<br /><br />The objective was to bring together entrepreneurs, undergraduate, and graduate students and professors to discuss new research challenges and strategies for different applications of natural polymers.<br />The event was attended by researchers from University of Araraquara (Uniara) - Araraquara, University of São Paulo (USP) - Pirassununga, São Paulo State University (UNESP) - Araraquara, and Federal University of São Paulo (Unifesp) - Diadema institutions, all located in the State of São Paulo.</div></div></div></div></div></div><div class="_1yuNDaQBDoy0YwHOMGXFkz"> </div></div><div class="_1BIi6rH1j5rnLh1OlF1NvI"><div class="_1XvPPaPlhtm-u0Wx9Efhd5"> </div></div> Several authors Copyright (c) 2020 International Journal of Advances in Medical Biotechnology - IJAMB 2020-02-10 2020-02-10 2 2 75 161 10.25061/2595-3931/IJAMB/2019.v2i2.44 Effects of grape seed extract on properties of type I collagen scaffolds https://journalamb.com/index.php/jamb/article/view/29 <p style="margin: 0cm 0cm 0pt; text-align: justify;"><span style="font-family: Times New Roman; font-size: medium;"> </span></p><p>To obtain a material with potential for use in tissue engineering, anionic collagen was obtained from porcine serosa (S) and bovine tendon (T) by alkaline hydrolysis for 72h. Part of this collagen was mixed with water to obtain 4 % (weight/weight) collagen suspension and part was solubilized in acetic acid pH 3.5 to obtain 1.5% (w/w) gel. The suspensions were mixed with their respective gels (2:1) (suspension: gel) and grape seed extract, whose main product is proanthocyanidin, was added at concentrations of 0.03% and 0.5%, thus obtaining the scaffolds SC (serosa collagen suspension and gel), TC (tendon collagen suspension and gel), SCP003 (SC with 0.03% extract), TCP003 (TC with 0.03% extract), SCP05 (SC with 0.5% extract added) and TCP05 (TC with 0.5% extract). The materials were analyzed by differential scanning calorimetry (DSC), scanning electron microscopy (SEM) and characterized by phosphate buffered saline absorption assay and in vitro biological stability assay. By DSC it is observed that the addition of 0.5% of extract increases the denaturation temperature (Td) of collagen, indicating that at this concentration the extract acts as polymer crosslinking agent. SEM shows disorganized cross-section pores in all scaffolds, not exceeding 130 ?m. Absorption and degradation assays indicated that the addition of 0.5% extract increases the absorption of phosphate buffered saline (PBS) by the scaffolds and decreases the degradation percentage by collagenase. These results suggests that the scaffolds can be used for different applications, e.g. as hemostatic agent.</p><p> </p><p class="Ttuloum"> </p> Claudio Fernandes Garcia Virginia C. A. Martins Ana M. G. Plepis Copyright (c) 2019 International Journal of Advances in Medical Biotechnology - IJAMB 2020-02-10 2020-02-10 2 2 02 10 10.25061/2595-3931/IJAMB/2019.v2i2.29 Effect of crosslinker and nanostructure on adsorption and release of paraquat herbicide from different natural hydrogel nanocomposites https://journalamb.com/index.php/jamb/article/view/36 <p><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Hidrogéis compostos em polímeros naturais que tenham sido usados em diferentes setores devido a suas propriedades de biocompatibilidade, biodegradabilidade, custo relativamente baixo e atoxicidade. </span></span></span><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">O objetivo deste estudo foi preparar hidrogéis de alginato / amido reticulados com diferentes ícones (Mn </span></span></span></span><sup><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">2+</span></span></span></span></sup><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"> , Zn </span></span></span></span><sup><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">2+</span></span></span></span></sup><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"> ou Ca </span></span></span></span><sup><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">2+</span></span></span></span></sup><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"> ), incluir zeólito ou nanoclay, e avaliar o efeito do reticador e nanoestrutura na adsorção e comportamento de seleção do herbicida paraquat. </span></span></span><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Os hidrogéis e seus nanocompósitos foram preparados por imersão de soluções de alginato / amido, alginato / amido / nanoclay ou alginato / amido / zeólita em recipientes que recebem uma solução reticuladora (MnCl </span></span></span></span><sub><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">2</span></span></span></span></sub><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"> , ZnCl </span></span></span></span><sub><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">2</span></span></span></span></sub><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">ou CaCl </span></span></span></span><sub><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">2</span></span></span></span></sub><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"> ) a 25 ° C. </span></span></span><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Todos os hidrogênios apresentaram boa capacidade de adsorção, principalmente os hidrogênios nanoestruturados com nanoclay e zeólito. </span></span></span><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Como recursos polares, selecione alta interação com moléculas de paraquat. </span></span></span><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">O comportamento da liberação também foi muito interessante, sendo que a quantidade de solução de formação de nanocompósitos de hidrogel pode controlar a liberação de paraquat, que tipos de materiais podem ser usados como veículos de transporte no sistema de liberação controlada.</span></span></span></span></p> Renan da Silva Fernandes Uilian Gabaldi Yonezawa Marcia Regina de Moura Fauze Ahmad Aouada Copyright (c) 2020 International Journal of Advances in Medical Biotechnology - IJAMB 2020-03-29 2020-03-29 2 2 11 18 10.25061/2595-3931/IJAMB/2019.v2i2.36 Surface-Modified Bacterial Cellulose with Mercaptosilane as a Multifunctional Platform https://journalamb.com/index.php/jamb/article/view/46 <h1>Cellulose synthesized by bacteria has unique properties such as high water retention capacity, biocompatibility, biodegradability and flexibility. Nevertheless, modification of this biomaterial is required in order to obtain multifunctional materials, which may be applied in several high-value added products, as catalytic and cell culture platforms. The surface of bacterial cellulose (BC) can be modified by several approaches, namely: (i) physical treatment by plasma, (ii) adsorption of molecules onto BC surface, and (iii) chemical modification. In this sense, the aim of this study was to modify the BC surface by silanization reaction at room temperature using a mixture of ethanol and water, using two different protocols. Thus, BC membranes synthesized by <em>Komagataeibacter xylinus </em>were modified by adding the thiol (SH) functional group with (3-mercaptopropyl)trimethoxysilane under mild conditions. The produced materials were analyzed by elemental analysis, ATR-FTIR, TGA and SEM, and the successful modification was proven by elemental analysis and SEM.</h1><p><strong> </strong></p> Nayara C. do Amaral Amanda M. Claro Gustavo C. Monteiro Hernane S. Barud Copyright (c) 2020 International Journal of Advances in Medical Biotechnology - IJAMB 2020-02-10 2020-02-10 2 2 19 24 10.25061/2595-3931/IJAMB/2019.v2i2.46 Effects of calcium phosphates incorporation on structural, thermal and drug-delivery properties of collagen:chitosan scaffolds https://journalamb.com/index.php/jamb/article/view/32 <p class="Estilo1"><span lang="EN-US">In this study, we evaluated how different procedures of calcium phosphate synthesis and its incorporation in collagen:chitosan scaffolds could affect their structural and thermal properties, aiming the obtention of homogeneous scaffolds which can act as drug delivery vehicles in bone tissue engineering. Therefore, three different scaffold preparation procedures were developed, changing the order of addition of the components: in CC-CNPM1 and CC-CNPM2, calcium phosphate synthesis was performed <em>in situ </em>in the chitosan gel (1%, w/w) followed by mixture with collagen (1%, w/w), with changes in the reagents used for calcium phosphate formation; in CC-CNPM3 procedure, calcium phosphate was synthesized <em>ex situ</em> and then incorporated into the collagen gel, in which chitosan in powder was mixed. In all procedures, 5% (in dry mass) of ciprofloxacin was incorporated. FTIR analysis confirmed the presence of calcium phosphate in all scaffolds. DSC curves showed that collagen denaturation temperature (Td) increased with calcium incorporation. SEM photomicrographs of scaffolds cross-section revealed porous scaffolds with calcium phosphate grains internally distributed in the polymeric matrix. XRD diffractograms indicated that the calcium phosphates obtained are hydroxyapatite. The pore size distribution was more homogeneous for CC-CNPM3, which also stood out for its smaller porosity and lower absorption in PBS. These results indicate that the <em>in situ </em>or <em>ex situ </em>phosphate incorporation in the scaffolds had a great influence on its structural properties, which also had consequences for ciprofloxacin release. CC-CNPM3 released a smaller amount of antibiotic (30%), but its release profile was better described by all the tested models. </span></p> Mirella Romanelli Vicente Bertolo Virginia Conceição Amaro Martins Ana Maria de Guzzi Plepis Copyright (c) 2020 International Journal of Advances in Medical Biotechnology - IJAMB 2020-02-10 2020-02-10 2 2 25 35 10.25061/2595-3931/IJAMB/2019.v2i2.32