International Journal of Advances in Medical Biotechnology - IJAMB 2021-03-18T09:16:23-03:00 Hernane Barud Open Journal Systems <p><strong>International Journal of Advances in Medical Biotechnology (IJAMB)</strong> is a peer-reviewed open access journal which provides a forum for discussion and dissemination of studies in Medical Sciences and Biotechnology fields. As a scientific publisher, IJAMB will focus on the dissemination of Original Papers, Reviews, Mini-reviews, Perspectives, Emerging Technologies and Spotlights relating scientific discoveries and inventions. The submitted papers should indicate the novelty and significant advances to the current state of knowledge and understanding of the proposed topic.</p> <p>In this context, the IJAMB emerged as a means of sharing new knowledge oriented towards to the future which is related to the most relevant advances on Medical Sciences and Biotechnology fields. Therefore, the topics of interest to the current journal include: Regenerative Medicine; Biomembranes and Biopolimers; Slow delivery systems of drugs; Medical devices; Medical chemistry organic and inorganic; 3D structures; Growth factors; Tissue Engineering; Electronic technologies for health applications and papers focusing on biological, physiological and pharmacological modelling molecules.</p> <p>As editorial guidance, the journal policy is based on the best practices of Brazilian and international scientific journals. Thus, the evaluation process adopted by the Journal is to publish peer-reviewed research which guarantees the confidentiality of authors and reviewers.</p> <p>The Editorial Board is committed to build up a journal with international recognition and adequate indexation based on international and national data. We consider to analize content articles as commissioned, but the Journal is also open to welcome new ideas. Please contact the Editorial Office ( with your proposal; once a proposal is accepted or commissioned, detailed format guidelines will be provided.</p> <p><img src="" alt="" width="700" height="990" /></p> Systematic review: the use of vaginal mold in current vaginoplasty surgeries - techniques and materials 2021-03-17T12:50:43-03:00 Jéssica Silveira Ávila Luanne Cardoso Mendes <p>This paper presents a study of the techniques and materials used in vaginoplasty surgeries performed around the world. It consists of a systematic review that covered the identification, selection and critique of primary studies of topics involving the use of vaginal molds in surgeries for different patients: women with Mayer-Rokitansky syndrome; and transgender patients who underwent sex reassignment surgery (CRS), that is, patients with gender dysphoria. The researches made in the chosen databases, after applying the criteria of inclusion and exclusion of articles, resulted in 19 publications, which represented the basis of the construction of this work. It also focuses on the description of the technologies, materials and methods used in the manufacture of vaginal molds used in surgery. In all studies, the molds have the function of maintaining the structure of the neovagina, thus avoiding vaginal stenosis, besides fixing the material used as a graft in the new cavity, covering it, favoring epithelization.</p> 2021-03-18T00:00:00-03:00 Copyright (c) 2021 International Journal of Advances in Medical Biotechnology - IJAMB A study on corrosion resistance of ISO 5832-1 austenitic stainless steel used as orthopedic implant 2021-03-17T13:32:09-03:00 Lilian N. M. Braguin Caio A. J. da Silva Larissa O. Berbel Isolda Costa Mitiko Saiki <p>The ISO 5832-1 austenitic stainless steel used as biomaterial is largely applied in the area of orthopedics, especially in the manufacture of implants, such as temporary or permanent replacement of bone structures. The objective of this study was to evaluate the localized corrosion resistance of the ISO 5832-1 stainless steel used in orthopedic implants by electrochemical tests in two different solutions. The results of this study are of great interest to evaluate the corrosion of metallic implants that can result in the release of corrosion products into bodily fluids causing possible adverse biological reactions. The determination of the chemical elements in the composition of the ISO 5832-1 stainless steel was performed by neutron activation analysis (NAA). The samples for electrochemical tests were grinded with silicon carbide paper up to #4000 finishing, followed by mechanical polishing with diamond paste. The open circuit potential measurements and anodic polarization curves were obtained in solution of 0.90 wt.&nbsp;% of NaCl and of simulated body fluid (SBF). The results indicated that the ISO 5832-1 stainless steel presented a high resistance to crevice corrosion in simulated body fluid solution but high susceptibility to this form of corrosion in the chloride solution.</p> <p><em>&nbsp;</em></p> 2021-03-18T00:00:00-03:00 Copyright (c) 2021 International Journal of Advances in Medical Biotechnology - IJAMB Simultaneous alterations in ovaries and bone as a result of Polycystic Ovary Syndrome 2021-03-13T11:44:38-03:00 Ana Lúcia de Oliveira Bonfá Eduardo Donato Alves Víctor Fabrício Keico Okino Nonaka Janete Aparecida Anselmo-Franci Jorge Alberto Achcar Luís Henrique Montrezor <p>Polycystic ovary syndrome (PCOS) is one of the most widely recognized endocrine disorders affecting reproductive-age women. The etiopathogenesis and mechanisms of this syndrome remain unclear. Diagnosis requires two of the following: polycystic ovaries, oligo- or anovulation, and hyperandrogenism. Most women with PCOS display conditions such as metabolic abnormalities, diabetes, obesity, cardiovascular disease, and/or bone dysfunction. Considering the ethical limitations of human studies, animal and cell culture models that reflect some features of PCOS are important for investigation of this syndrome. The aim of the present work was to study some of the endocrine relationships between ovaries and bone tissue in a polycystic ovary syndrome animal model. The study was performed using an estradiol valerate PCOS-induced rat model (n = 30) and bone mesenchymal stem cell cultured from bone marrow of those animals. It was hypothesized that changes of the endocrine relationship between ovaries and bones could be observed in from <em>in vivo</em> animal model and <em>in vitro</em> cell culture assays. The ovarian morphological and endocrine changes seem to be correlated with endocrine, biophysical, and biomechanical changes in bone properties. Mesenchymal stem cells obtained from PCOS-induced rats, cultured for up to 21 days and differentiated into osteoblasts, presented lower viability and reduced mineralization of the extracellular matrix. Taken together, these results indicate important endocrine and structural effects of PCOS in ovaries and bones, contributing to part of the understanding of the pathophysiological mechanisms of PCOS.</p> 2021-03-18T00:00:00-03:00 Copyright (c) 2021 International Journal of Advances in Medical Biotechnology - IJAMB Study of ph effect on AZ31 magnesium alloy corrosion for using in temporary implants 2020-12-04T18:37:30-02:00 Caio A. J. da Silva da Silva Lilian N. M. Braguin Larissa O. Berbel Bárbara V. G. de Viveiros Jesualdo L. Rossi Mitiko Saiki Isolda Costa <p><em>Currently, magnesium alloys are gaining great interest for medical applications due to their degrading properties in the human body ensuring a great biocompatibility. These alloys also provide profitable mechanical properties due similarities with human bone.&nbsp; However, a difficulty in applying these materials in the biomaterials industries is the corrosion prior to cell healing. The effect of the chemical composition of Mg alloys on their corrosion behavior is well known. In this study, samples of AZ31 magnesium alloy were cut into chips for elemental chemical analysis by neutron activation analysis (NAA). Concentrations of the elements As, La, Mg, Mn, Na, Sb and Zn were determined in the AZ31 alloy. Visualization tests of agar corrosion development in various media, of 0.90% sodium chloride solution (mass), phosphate buffer saline (PBS) and simulated body fluid (SBF) were performed. Visualizations of the effect of agar gel corrosion revealed pH variation during the corrosion process due to the released into the cathode. The highest released of hydroxyl ions occurred in NaCl solution compared to PBS and SBF solutions indicating that NaCl solution was much more aggressive to the alloy compared to the others.</em></p> 2021-04-14T00:00:00-03:00 Copyright (c) 2021 International Journal of Advances in Medical Biotechnology - IJAMB Productionof poly(L-CO-D,L LacticAcid) porous fibers by electrospinning 2020-09-22T23:34:47-03:00 Nayara Maysa da Silva Carvalho Bárbara E. Ciocca Rubens Maciel Filho Marcele Fonseca Passos Maria Regina Wolf Maciel Rogerio Furlan <p>The production of porous scaffolds has been widely investigated by the scientific community due to its suitability for tissue engineering. Among techniques that allow the fabrication of porous materials, electrospinning is appealing for being robust and versatile. This research investigated the pore formation in poly (L-co-D,L lactic acid) fibers obtained by conventional electrospinning and the influence of chloroform as a single solvent on fiber morphology. Random and highly porous fibers with a mean diameter of 2.373 ± 0.564 µm were collected. Chloroform affects the fiber morphology, mainly for its fast evaporation and low density of charges. The solvent on the surface evaporates quickly, and the low stretch of the jet does not help the polymer to reorganize over the length of the fiber, forming pores. In conclusion, the low dielectric constant and boiling point of chloroform induce pores formation along the PLDLA fibers.</p> <p><em> </em></p> 2021-03-18T00:00:00-03:00 Copyright (c) 2021 International Journal of Advances in Medical Biotechnology - IJAMB Ibuprofen nanocrystals: Production, lyophilization and release profile 2020-01-13T14:49:14-02:00 Patricia Severino Taline Almeida Thallysson Barbosa Carolina Rodrigues Marina Costa João Ferreira João Dias-Ferreira Ana Fernandes Eliana Souto <p>Ibuprofen (IBU) is a poorly water soluble non-steroidal anti-inflammatory drug with proven effectiveness for treating inflammatory, musculoskeletal, and rheumatic disorders. Nanocrystals (NCs) have been proposed as drug delivery systems to improve the solubility and bioavailability of poorly water-soluble compounds. Ibuprofen NCs (IBU-NCs) have been produced by melt-emulsification method using a combination of Tween<sup>®</sup>80/Span<sup>®</sup>80 as surfactant as these molecules are generally recognized as safe (GRAS) as non-toxic, non-irritating and are of low cost. The obtained main particle size (z-Ave) and polydispersity index (PdI) were 159.4 ± 3.265 nm and 0.24 ± 0.007, respectively. Lyophilization slightly increased the mean particle size and PdI compared to the non-freeze-dried IBU-NCs. The obtained IBU-NCs powders were of white and fine texture. The type and concentration of cryoprotector (trehalose, glucose, sucrose) influenced both the size and the <em>in vitro</em> release profile tested in Franz diffusion cells. Due to the smaller z-Ave, NCs:Trehalose (2:1) of 170.6 ± 3.880 nm (0.417 ± 0.050), NCs:Glucose (3:1) of 275.3 ± 8.351 nm (0.144 ± 0.021) and NCs:Sucrose (4:1) of 223.3 ± 10.35 nm (0.402 ± 0.016) were selected for the in vitro drug release tests. Within the first 6 hours, resuspended lyophilized nanocrystals released between 50-70% of the drug.</p> 2021-03-18T00:00:00-03:00 Copyright (c) 2021 International Journal of Advances in Medical Biotechnology - IJAMB