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Mini-review

Vol. 4 No. 2 (2021): October-February

Comparison between three decellularization protocol whit SDS in swine esophagus: a future option in veterinary medicine

DOI
https://doi.org/10.52466/ijamb.v4i2.92
Published
2021-10-10

Abstract

Disease such as megaesophagus and esophagitis does not have a cure. Esophagitis has treatments depending on it’s degree, while megaesophagus has a palliative treatment, which can be recommended euthanasia in cases of a poor prognosis, cases that the esophagus transplant could be at use, although it is not described in literature. In this perspective the regenerative medicine have techniques of a bioengineering of tissues, creating a minor immune response in transplants, as it removes the organ’s genetic material, preserving the extra cellular matrix (ECM). Different protocols have been evaluated for swine esophagus decellularization, using sodium dodecyl sulphate (SDS) in concentrations 0,5%, 1,0% and 1,5% for seven days, analysing the remaining EMC integrity. It has been concluded that the material in 0,5% of SDS showed more integrity of the remaining EMC compared to the other concentrations, demonstrating that it is the best biological scaffold for future medical applications.  

References

  1. - LITTLE, Susan. The cat: INTERNAL MEDICINE. [S.L.]: ABDR, 2012.
  2. - SELLON, Rance K .; WILLARD, Michael D. Esophagitis and esophageal strictures. Veterinary Clinics: Small Animal Practice, v. 33, no. 5, p. 945-967, 2003.
  3. - TAMS, T. R. Esophageal diseases. TAMS, TR Small Animal Gastroenterology, v. 2, p. 115-153, 2005.
  4. - NELSON, Richard; COUTO, C. Guillermo. Internal medicine of small animals. Elsevier Brazil, 2015.
  5. - FOSSUM, T. H. et al. Digestive system surgery. Small animal surgery, p. 222-405, 2002.
  6. - SLATTER, Douglas H. (Ed.). Textbook of small animal surgery. Elsevier Health Sciences, 2003.
  7. - DE SOUZA FILHO, Zacarias Alves et al. Use of porcine small intestine submucosa in the repair of cervical esophageal lesions. Experimental study in dogs1. Brazilian Surgical Act, v. 18, no. 3, 2003.
  8. - SANTOS, Carlos Eduardo Meirelles of the. Analysis of thoracic esophageal removal and replacement: experimental study in dogs. 2008.
  9. - OZEKI, Masayasu et al. Evaluation of decellularized esophagus as a scaffold for cultured esophageal epithelial cells. Journal of Biomedical Materials Research Part A, v. 79, no. 4, p. 771-778, 2006.
  10. - CRAPO, Peter M .; GILBERT, Thomas W .; BADYLAK, Stephen F. An overview of tissue and whole organ decellularization processes. Biomaterials, v. 32, no. 12, p. 3233-3243, 2011.
  11. - GILBERT, Thomas W .; SELLARO, Tiffany L .; BADYLAK, Stephen F. Decellularization of tissues and organs. Biomaterials, v. 27, no. 19, p. 3675-3683, 2006.
  12. - BADYLAK, Stephen F .; TAYLOR, Doris; UYGUN, Korkut. Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds. Annual review of biomedical engineering, v. 13, p. 27-53, 2011.
  13. - WALLIS, John M. et al. Comparative assessment of detergent-based protocols for mouse lung de-cellularization and re-cellularization. Tissue Engineering Part C: Methods, v. 18, no. 6, p. 420-432, 2012.
  14. - KEANE, TJ; BADYLAK, SF (2015), The host response to allogeneic and xenogeneic biological scaffolding materials. J Tissue Eng Regen Med, 9, 504-511. doi: 10.1002 / term.1874.
  15. - BANCROFT, John D .; GAMBLE, Marilyn (Ed.). Theory and practice of histological techniques. Elsevier Health Sciences, 2008.
  16. - König, Horst Erich; Liebich, Hans-Georg. Anatomy of Domestic Animals-: Text and Colorful Atlas. Artmed Publisher, 2016.
  17. - DYCE, Keith M .; WENSING, Cornelius JG; SACK, Wolfganf O. Veterinary Anatomy Treaty. Elsevier Brazil, 2004.
  18. - JUNQUEIRA, Luiz C .; CARNEIRO, José. Basic Histology: Text / atlas. 11th ed. Rio de Janeiro: Guanabara, 2012. 50-316 p.
  19. - NETO, Abel Dorigan. TECHNIQUE IN CYTOPATHOLOGY: Reference Book 3: Histopathology Techniques. Reference Book 3, Brasília-DF, v. 1, no. 1, p. 36, Nov. 2012. Available at: <http://bvsms.saude.gov.br/bvs/publicacoes/tecnico_citopatologia_caderno_referencia_3.pdf>. Accessed on: Oct 25 2019.
  20. - PEREIRA, ANTONIO L.A., VERAS, SIMONE S.L., SILVEIRA, ÉRICKA J.D., SAABRA, FLÁVIO R.G. The role of extracellular matrix proteins and metalloproteinases in head and neck carcinomas: a bibliographic update. Brazilian Journal of Otorhinolaryngology, 71 (1), 81-86. https://dx.doi.org/10.1590/S0034-72992005000100014
  21. - ACKBAR, Richard et al. Decellularized ovine esophageal mucosa for esophageal tissue engineering. Technology and Health Care, v. 20, n. 3, p. 215-223, 2012.
  22. - SITTHISANG, Sonthikan; LEONG, Meng Fatt; CHIAN, Kerm Sin. Perfusion decellularization of porcine esophagus: Study of two processing factors affecting the folded mucosal structure of the esophageal scaffold. Journal of Biomedical Materials Research Part A, v. 109, n. 5, p. 745-753, 2021.

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