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Review

Vol. 4 No. 1 (2021): March-September

Evaluation of decellularization of porcine pericardium: Decellularization of porcine pericardium

DOI
https://doi.org/10.52466/ijamb.v4i1.87
Submitted
June 4, 2021
Published
2020-03-01

Abstract

The porcine pericardium has been used for its great potential as a biological scaffold, produced from the extracellular matrix (ECM) and used mainly in surgeries reconstructive, tissue repair and surgical procedures for corneal reconstruction. The adequate preservation and biocompatibility of the pericardial ECM structure during the decellularization process is fundamental, the biggest challenge being the total removal of cellular material without damage to the structure. All agents used in decellularization change the composition and cause some damage to the ultrastructure. Sodium Dodecil Sulfate (SDS) is the most effective for removing cell residue from tissue compared to other detergents, which is also the most used for the decellularization process. This work aimed to test 3 different concentrations of SDS, in order to assess the concentration (0.1, 0.5 and 1%) that best preserves the structure of the ECM pericardial. In addition, we listed the type of daily wash to make the process more effective (only distilled water or PBS 1x), in order to assess the concentration capable of decellularizing the tissue and better preserving the pericardial ECM. The concentration of SDS at 1%, when compared to the lowest concentrations of 0.1 and 0.5%, was more effective in the decellularization process, however it did not obtain good results in the preservation of the ECM. Regarding daily washing, there was no difference in the frequency assessed in the experimental groups.

References

  1. Heuschke MA, Leitolis A, Roderjan, JG, Suss PH, Luzia CAO, Da Costa FDA, Correa A, Stimamiglio MA. In vitro evaluation of bovine pericardium after a soft decellularization approach for use in tissue engineering. Xenotransplantation, e12464., 2018. doi:10.1111/xen.12464.
  2. Bender HW, Stewart JR, Merrill WH, Hammon JW, Graham TP.(1989), “Ten years’ experience with the senning operation for transposition of the great arteries: physiological results and late follow-up”, department of cardiac and thoracic surgery and division of pediatric cardiology, vanderbilt university medical center, nashville, tennessee, v. 47, p. 218–223.
  3. Taylor DA., Frazierb OH, Elgaladb A, Mendeza CH, Sampaioa LC. (2018), “Building a total bioartificial heart: harnessing nature to overcome the current hurdles”, Regenerative medicine research, Texas heart institute, Houston.
  4. Baucia JA, Leal Neto RM, Rogero JR e Nascimento N. 2006. Tratamentos anticalcificantes do pericárdio bovino fixado com glutaraldeído: comparação e avaliação de possíveis efeitos sinérgicos. Revista Brasileira de Cirurgia Cardiovascular. 21 (2): 180-187. doi.org/10.1590/S0102-76382006000200011
  5. Oréfice RL, Pereira MM, Mansur HS. (2006). “Biomateriais: fundamentos e aplicações”, Cultura médica, São Paulo, p. 538.
  6. Fu RH, Wang YC, Liu SP, Shih TR, Lin HL, Chen YM, Sung, JH, Lu CH, Wei JR, Wang ZW, Huang SJ, Tsai CH, Shyu WC, Lin SZ. Decellularization and Recellularization Techonologies in Tissue Engineering. Cell Transplantation, USA, v. 23, n. 4-5, p. 621-630, 2014.doi.org/10.3727/096368914X678382.
  7. Crapo PM, Gilbert TW, Badylak SF. (2011), “An overview of tissue and whole organ decellularization processes”, Biomaterials, elsevier, v.32, p. 3233-3243. doi: 10.1016 / j.biomaterials.2011.01.057.
  8. Gálvez-Montón C, Prat-Vidal C, Roura S, Soler-botija C, Bayes-Genis A. (2013), “Cardiac tissue engineering and the bioartificial heart”, Sociedad espanõla de cardiología, elsevier, v. 66, p. 391-399. doi: 10.1016 / j.rec.2012.11.012.
  9. Grauss RW, Hazekamp MG, Oppenhuizen F, Munsteren CJV, Groot ACG, Deruiter MC (2005), “Histological evaluation of decellularised porcine aortic valves: matrix changes due to different decellularisation methods”, European Journal of Cardio-Thoracic Surgery, v. 27, p.566–571. doi: 10.1016/j.ejcts.2004.12.052.
  10. Tolosa EMC, Rodrigues CJ, Behmer OA, Neto AG. Manual de Técnicas para Histologia Normal e Patológica. 1 ed, Manole, 2003.
  11. Mallis P, Michalopoulos E, Dimitriou C, Kostomitsopoulos N, Stavropoulos-Giokas C (2017). “Histological and biomechanical characterization of decellularized porcine pericardium as a potential scaffold for tissue engineering applications”, Bio-medical materials and engineering, 28(5), 477–488.
  12. Wollmann L, Suss P, Mendonça J, Luzia C, Schittini A, George WX., Costa F, Tuon, FF. (2019). Characterization of Decellularized Human Pericardium for Tissue Engineering and Regenerative Medicine Applications. Arquivos Brasileiros de Cardiologia–. doi:10.5935/abc.20190094
  13. Oswal D, Korossis S, Mirsadraee S, Wilcox H, Watterson K, Fisher J, Ingham E. (2007). “Biomechanical characterization of decellularized and cross-liked bovine pericardium”, J. heart valve dis. 16, 165–174.
  14. Chang Y, Lee M, Liang H, Hsu C, Sung H. (2004), “Acellular bovine pericardia with distinct porous structures fixed with genipin as an extracellular matrix”, Tissue eng. 10, 881–892.
  15. Dong J, Li Y, Mo X. (2012), “The study of a new detergent (octyl-glucopyranoside) for decellularizing porcine pericardium as tissue engineering scaffold”, Journal of surgical research, 183(1), 56–67. doi: 10.1016 / j.jss.2012.11.047.
  16. Zhou J, Fritze O, Schleicher M, Wendel HP, Schenke-Layland K, Harasztosi C, Shengshou H, Stock UA. (2009). “Impact of heart valve decellularization on 3-d ultrastructure, immunogenicity and thrombogenicity”, Biomaterials, 31(9), 2549–2554. doi:10.1016/j.biomaterials.2009.11.088
  17. Lopera Higuita M, Griffiths LG. (2020). Antigen removal process preserves function of small diameter venous valved conduits, whereas SDS-decellularization results in significant valvular insufficiency. Acta Biomaterialia. doi:10.1016/j.actbio.2020.03.003.
  18. Cheng J, Li J, Cain Z, Xing Y, Wang C, Guo, L, Gu, Y. (2020). Decelularização das artérias carótidas suínas usando dodecilsulfato de sódio em baixa concentração. The International Journal of Artificial Organs, (), 039139882097542–. doi: 10.1177 / 0391398820975420.
  19. Lee TC, Midura RJ, Hascall VC, Vesely I. The effect of elastin damage on the mechanics of the aortic valve. J. Biomech. 34:203–210, 2001. doi: 10.1016 / s0021-9290 (00) 00187-1.

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