Bacterial cellulose (BC) is a biopolymer of great interest for application in various industrial and medical areas, due to its peculiar characteristics of biocompatibility, tensile strength, high water retention capacity and crystallinity. CB can be produced using culture media with different nutrient sources, under static or agitated cultivation conditions. The objectives of this work were to produce CB membranes, using the bacterial strain Komagateibacter hansenii ATCC 23769, in static cultivation, using media containing different sources of carbon (glucose, fructose, glycerol and ethanol) and nitrogen (yeast extract and peptone), aiming to study the influence on the physicochemical properties of the BC produced and its possible biomedical application. The obtained BC were comparatively evaluated for dry mass yield, swelling capacity, and characterized by Field emission gun-scanning electron microscopy (FEG-SEM), and Fourier transform spectroscopy (FTIR). Membranes with significant swelling capacity were obtained, demonstrating that the composition of the culture medium influences the physicochemical properties of BC. The results of this work also demonstrate that the BC has great possibilities in several areas, including biomedical applications.